Effect of Arbuscular Mycorrhizal Fungi (AMF) on the Yields of Pepper (Capsicum annum L.) under Protected Conditions

Main Article Content

Beatriz Toledo Cabrera
https://orcid.org/0000-0002-3852-186X
Gerardo Montero Limonta
Anieska Bazán Delgado
https://orcid.org/0000-0003-3814-6999

Resumen

Context: Pepper yields have declined in recent years due to the impact of several factors, including the presence and little availability of nutrients in the soil, and the lack of efficient strains of AMF in the technological package. The application of AMF strains in the nutritional process of pepper improves nutrient availability in the soil, and increases crop yields.


Aim: To evaluate the yields of pepper (Capsicum annum L.) inoculated with efficient strains of arbuscular mycorrhizal fungi (AMF)


Methods: Direct inoculation of microorganisms during transplantation with a 10% proportion, depending on the root-ball volume. A completely randomized experimental design was used, with four treatments, and four repetitions, with a control that was not inoculated, and three levels of inoculation with Rhizophagus intraradices (INCAN-11), Glomus cubense (INCAN-4), and Funneliformis mosseae (INCAN-2). The zigzag sampling of fungal variables was done along the field, and several variables were evaluated: root colonization percent, visual density, and spore contents. Additionally, plant and fruit height and thickness were determined, and the fruits were weighed to determine crop yields.


Results: The best results were achieved with the Glomus cubense (INCAN-4) (T3) strain. The results observed showed the efficacy of the third treatment, which produced a cost-effectiveness of $ 3.13.


Conclusions: The AMF strains used had a favorable behavior in relation to the indicators evaluated: growth, development, and crop yield. The application of G. cubense (INCAN-4) produced the best pepper (Capsicum annum L.) yields.

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Resumen

Context: Pepper yields have declined in recent years due to the impact of several factors, including the presence and little availability of nutrients in the soil, and the lack of efficient strains of AMF in the technological package. The application of AMF strains in the nutritional process of pepper improves nutrient availability in the soil, and increases crop yields.


Aim: To evaluate the yields of pepper (Capsicum annum L.) inoculated with efficient strains of arbuscular mycorrhizal fungi (AMF)


Methods: Direct inoculation of microorganisms during transplantation with a 10% proportion, depending on the root-ball volume. A completely randomized experimental design was used, with four treatments, and four repetitions, with a control that was not inoculated, and three levels of inoculation with Rhizophagus intraradices (INCAN-11), Glomus cubense (INCAN-4), and Funneliformis mosseae (INCAN-2). The zigzag sampling of fungal variables was done along the field, and several variables were evaluated: root colonization percent, visual density, and spore contents. Additionally, plant and fruit height and thickness were determined, and the fruits were weighed to determine crop yields.


Results: The best results were achieved with the Glomus cubense (INCAN-4) (T3) strain. The results observed showed the efficacy of the third treatment, which produced a cost-effectiveness of $ 3.13.


Conclusions: The AMF strains used had a favorable behavior in relation to the indicators evaluated: growth, development, and crop yield. The application of G. cubense (INCAN-4) produced the best pepper (Capsicum annum L.) yields.

Article Details

Cómo citar
Toledo Cabrera, B., Montero Limonta, G., & Bazán Delgado, A. (2020). Effect of Arbuscular Mycorrhizal Fungi (AMF) on the Yields of Pepper (Capsicum annum L.) under Protected Conditions. Agrisost, 26(1), 1-11. https://doi.org/10.5281/zenodo.7595309
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Agrobiodiversidad

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Alonso, R, Aguilera, L. I., Rubí, M., González, A., Olalde, V., & Rivas, I. V. (2013). Influencia de hongos micorrízicos arbusculares en el crecimiento y desarrollo de Capsicum annuum L. Revista Mexicana en Ciencias Agrícolas, 4(1), 77-88. Retrieved on May 20, 2018, from: http://www.scielo.org.mx/pdf/remexca/v4n1/v4n1a6.pdf
Angulo, A., Ferrera, R., Alarcón, A., Almaraz, J. J.., Delgadillo, J., Jiménez, M., & García, O. (2018). Crecimiento y eficiencia fotoquímica del fotosistema ii en plántulas de 2 variedades de Capsicum annuum L. inoculadas conrizobacterias u hongos micorrízicos arbusculares. Revista Argentina de Microbiología, 50(2), 178-188, doi: https://doi.org/10.1016/j.ram.2017.03.011.
Bell-Mesa, T. D., Osoria-Galan, D., Montero-Limonta, G., & Molina-Lores, L. B. (2017). Efecto de hongos micorrícicos arbusculares sobre Pimiento (Capsicum annum L.) en la producción de plántulas en campo antena, santiago de cuba. Ciencia en su PC, (4), 53-67. Retrieved on December 5, 2019, from: https://www.redalyc.org/articulo.oa?id=181353794004
Casanova Morales, A., Gómez, O., Pupo, F. R., Hernández, M., Chailloux, M., Depestre, T., … Moreno, V. (2007). Manual para la producción Protegida de hortalizas. La Habana, Cuba: Ministerio de la Agricultura; Viceministerio de Cultivo Varios; Instituto de Investigaciones Hortícolas Liliana Dimitrova.
Charles, N. J., & Martín, N. J. (2015). Uso y manejo de hongos micorrízicos arbusculares (HMA) y humus de lombriz en tomate (Solanum lycopersicum L.), bajo sistema protegido. Cultivos Tropicales, 36(1), 53-62. Retrieved on April 4, 2019, from: http://scielo.sld.cu/pdf/ctr/v36n1/en_ctr07115.pdf
Gamboa, L. A. (2015). Analizar la diversidad morfológica de los hongos micorrízico sarbusculares (HMA) presentes en la rizósfera de Larrea Tridentata. (Tesis para obtener el título de Ingeniero en Agroecología, Universidad Autónoma Agraria Antonio Narro. Unidad Laguna). Retrieved on January 5, 2019, from: http://repositorio.uaaan.mx:8080/xmlui/bitstream/handle/123456789/6969/LUIS%20ALEXIS%20GAMBOA%20CRUZ.pdf?sequence=1
García, A, G., Hernández, N., Méndez, V., Toscano, M., Mulling, M., Mosquera, D., & Chong (2007). Genotipos de frijol con uso eficiente del fósforo. En Memoria del Taller Regional sobre Manejo de la Nutrición del Frijol Común en Condiciones de Estrés. (56p.). Cali Colombia: Centro Internacional de Agricultura Tropical (CIAT).
Gerdemann, J. W., & Nicolson, T. H. (1963). Spore of mycorrhizae endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc., 46(2), 235-244, doi: https://doi.org/10.1016/S0007-1536(63)80079-0
Giovanetti, M., & Mosse, B. (1980). An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol, 84, 489-500. Retrieved on February 12, 2019, from: https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1469-8137.1980.tb04556.x
González, P. J., Arzola, J., Morgan, O., Rivera, R., Plana, R., & Fernández, F. (2008). Manejo de las asociaciones micorrízicas en pastos del género Brachiaria, cultivados en suelos ferralítico rojo y pardo mullido. Congreso Científico del Instituto Nacional de Ciencias Agrícolas (INCA). [CD-ROOM]. La Habana, Cuba: INCA.
Gregersen, H. M., & Contreras A. R. (1980). Análisis económico de proyectos forestales. Roma: FAO. Retrieved on May 12, 2019, from: http://www.fao.org/3/a-ap346s.pdf
Herrera R. A., Ferrer R. L., Furrazola E., & Orozco M. O. (1995). Estrategia de funcionamiento de las micorrizas VA en un bosque tropical. Biodiversidad en Iberoamérica. Ecosistemas, Evolución y Procesos sociales. En M. Monasterio (Eds.), Programa Iberoamericano de Ciencia y Tecnología para el desarrollo. Subprograma XII, Diversidad Biológica, Mérida, Colombia.
Jiménez Montejo, G., Ramírez Núñez, Y., & Mena Campos, J. (2014). Selección de rizobacterias por su antagonismo frente a microorganismos patógenos de cucurbitáceas. Agrisost, 20(2), 1-16. Retrieved on May 20, 2018, from: https://revistas.reduc.edu.cu/index.php/agrisost/article/view/334
Jiménez Montejo, G., Mena Campos, J., & Ramírez Núñez, Y. (2014). Control biológico de enfermedades mediante el tratamiento de semillas de cucurbitáceas con rizobacterias promotoras del crecimiento de las plantas (PGPR). Agrisost, 20(1), 12-27. Retrieved on May 20, 2018, from: https://revistas.reduc.edu.cu/index.php/agrisost/article/view/338
Martínez, V., Dibut, B., & Ríos, Y. (2010). Efecto de la integración de aplicaciones agrícolas de biofertilizantes y fertilizantes minerales sobre las relaciones suelo-planta. Cultivos Tropicales, 31(3), 27-31. Retrieved on May 20, 2018, from: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0258-59362010000300009
Montero, L., Duarte, C., Cun, R., & Cabrera, J. A. (2010). Efectividad de biofertilizantesmicorrízicos en el rendimiento del pimiento (Capsicumannuum L. var. Verano 1) cultivado EN diferentes condiciones de humedad del sustrato. Cultivos Tropicales, 31(3). Retrieved on March 18, 2019, from: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0258-59362010000300001&lng=es&tlng=es
ONEI. Oficina Nacional de Estadística e información. (2016). Anuario Estadistico de Santiago de Cuba. Edición 2017. Retrieved on January 20, 2019, from: http://www.onei.gob.cu/sites/default/files/anuario_est_provincial/00_santiago_de_cuba.pdf
Paneque, V. M., Calaña, J. M., Calderón, M., Borges, Y., Hernández, T. C., & Caruncho, M. (2010). Manual de Técnicas Analíticas para análisis de suelo, foliar, abonos orgánicos y fertilizantes químicos. La Habana: INCA. Retrieved on March 12, 2019, from: http://ediciones.inca.edu.cu/files/folletos/folleto_suelos.pdf
Phillips, J. M., & Hayman, D. S. (1970). Improved procedures for clearing roots and staining parasitic and vesicular arbuscularmycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55(1), 158-161, doi: https://doi.org/10.1016/S0007-1536(70)80110-3
Reche, J. (2010). Cultivo del pimiento dulce en invernadero. Sevilla: Consejería de Agricultura y Pesca, Servicio de Publicaciones y Divulgación. (Agricultura. Estudios e informes técnicos). Retrieved on March 18, 2019, from: https://www.juntadeandalucia.es/export/drupaljda/1337160265Cultivo_Pimiento_Invernadero.pdf
Rivera, G., Martínez, M.A., Vallejo, S., Alvarez, G., Varga, I., Moya P., & Yúfera, E. (2001). In vitro inhibition of mycelial growth of Tilletiaindica by extracts of native plants from Sonora, Mexico. Revista Mexicana de Fitopatología. Retrieved on February 16, 2019, from: http://www.redalyc.org/articulo.oa?id=61219213
Rivera, R., Fernández, F., Fernández, K., Ruiz, L., Sánchez, C. & Riera, M. (2007). Advances in the management of effective arbuscular symbiosis in tropical ecosystems. In: Mycorrhizae in Crop Productions. Hamel, C. y Plenchette, C. (Eds.). The Haworth Press, Inc. USA. Retrieved on February 16, 2019, from: https://www.researchgate.net/profile/Ramon_Espinosa/publication/269993713_Advances_in_the_management_of_effective_arbuscular_mycorrhizal_symbiosis_in_tropical_ecosystesm/links/550c4e3f0cf2ac2905a3c2fb.pdf
Rivera, R., Ruíz, L., Martín, G., Pérez, E., Nápoles, M. C., Garcías, M., et al. (2015). ANEXO INFORME PRIMER SEMESTRE/JUNIO 2015 DEL MEGAPROYECTO. “Manejo conjunto e impacto de biofertilizantes micorrízicos y otros bioproductos en la producción agrícola de diferentes cultivos”. CÓDIGO: P131LH0010003. Retrieved on January 16, 2020, from: https://www.researchgate.net/publication/294582507_Manejo_conjunto_e_impacto_de_biofertilizantes_micorrizicos_y_otros_bioproductos_en_la_produccion_agricola_de_diferentes_cultivos
Rivera, P. J. González, A., Hernández; G., Martín, Ruíz, L., Fernández, K., … Ruiz, M. (2015). La importancia del ambiente edáfico y del pH sobre la efectividad y la recomendación de cepas eficientes de HMA para la inoculación de los cultivos. Retrieved on February 16, 2019, from: https://www.researchgate.net/publication/279193397_La_importancia_del_ambiente_edafico_y_del_pH_sobre_la_efectividad_y_la_recomendacion_de_cepas_eficientes_de_HMA_para_la_inoculacion_de_los_cultivos
Rodríguez, Y., Dalpé, Y., Séguin, S., Fernández, F., Fernández, F., & Rivera, R. (October–December 2011). Glomus cubense sp. nov., anarbuscular mycorrhizal fungus from Cuba. MYCOTAXON, 118, 337-347, doi: https://doi.org/10.5248/118.337
Ruth, B., Khalvati, M., & Schmidhalter, U. (2011). Quantification of mycorrhizal water uptake via high-resolution on-line water content sensors. Plant Soil, 342(1-2), 459–468, doi: https://doi.org/10.1007/s11104-010-0709-3
Schüβler, A., & Walker, C. (2011). Evolution of Fungi and Fungal-Like Organisms (1st ed., Vol. 14, pp. 164-185). (The Mycota: A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research). Heidelberg, Germany: Springer. Retrieved on February 15, 2019, from: https://research-repository.uwa.edu.au/en/publications/evolution-of-the-plant-symbiotic-fungal-phylum-glomeromycota
Trouvelot, A., Kough, J., & Gianinazzi, V. (1986). Mesure du taux de mycorhization VA d'un systemeradiculaire. Recherche de methods d'estimation ayantune signification fonctionnelle. En Physiological and genetical aspects of mycorrhizae: proceedings of the 1st european symposium on mycorrhizae, Dijon. (pp. 217-221). Paris: INRA.